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Using genome mining, NcEgt2, a pyridoxal phosphate (PLP)-binding cysteine desulfurase enzyme that converts hercynylcysteine sulfoxide to EGT, was identified and the C-S lyase activity of Egt2 (NCU11365) to generate EGT was reconstituted in vitro (31) (Fig

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However, the disappearance of GSH was not accounted for by its conversion to GSSG as this form was not detected in the representative HPLC chromatogram shown