After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader
A.HoffmannP
Plant Cell 11 (6): 1153-1164
ACC 57) at passage 425 were cultured in Dulbeccos Modified Eagle Medium (DMEM) (Gibco, Ref: 41966029, Thermofisher Scientific,Waltham, Massachusetts, United States) supplemented with 10% foetal bovine serum (FBS) (Gibco, Ref: 10270106, Thermo Fisher Scientific, Waltham, Massachusetts, United States) in humidified air at 37 C and 5% CO 2
Adjust BAC water volume to make the draw easy to read on your syringe