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Membranes were blocked in 5% non-fat milk in tris-buffered saline with 0.1% Tween (TBST) overnight at 4C and subsequently incubated with primary antibodies in 5% BSA in 1X TBST overnight at 4C

For assessment of the nuclear cytoplasmic ratio of Nrf2 signal intensity, the nucleus was defined by Hoechst H-33342 staining

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For medical use, see Cyanocobalamin injection for adverse effects and precautions