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The assay was conducted at 37 C, and fluorescence intensity (reflecting the release of free rhodamine G upon Ub-RhoG cleavage by active DUBs) was measured at an excitation wavelength of 492 nm and an emission wavelength of 525 nm at 20-min intervals over a total of 1 h (0, 20, 40, and 60 min)

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593,594 M2-polarized macrophages show increased production of itaconate, which supports their anti-inflammatory function