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Soluble fractions were separated from denatured proteins by ultracentrifugation at 100,000 g and 4 C for 20 min (Beckman Coulter Optima MAX-XP, with TLA-120.1 rotor)

NHS access requires meeting NICE criteria: BMI 35 kg/m (or 32.5 kg/m for certain ethnic groups) with weight-related comorbidities, referral to specialist weight management services, and demonstrated response to treatment at defined review points

This dual mechanism may contribute to greater reductions in HbA1c and body weight in clinical trials

Calcium is important for bone health, muscle function, and nerve signalling, though it is less commonly depleted through gastrointestinal losses

Supplementary Figs S5 and S6 and Supplementary methods)