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After incubation, cells were stained with propidium iodide (PI, # 40710ES03, Yeasen) solution (40 g/mL in PBS) for 15 min at room temperature and the cell cycle was analyzed using Flowsight flow cytometer (Merck Millipore, Darmstadt, Germany)

In addition, it can also help with cardiovascular disease and weight loss via its mechanism

Both of these injection sites contain major arteries (the jugular and femoral, respectively), and it can be deadly to pierce them by mistake

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