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Moisten a cotton ball with rubbing alcohol and use it to wipe the skin at the injection site

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However, once you reconstitute the vial using bacteriostatic water, the shelf life is drastically reduced

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Immunofluorescence results showed that EA effectively activated GLP-1 neurons in the NTS, and the number of activated GLP-1 neurons decreased significantly after chemogenetic suppression, demonstrating that the chemogenetic tools virus was functioning properly (Fig