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Zhou X et al
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To visualize transfected cells, the slides were incubated with FLAG-tag antibody (1:1000) for 1 h at room temperature, washed 3 times for 10 min in TBS plus 0,05% Tween-20, and subsequently incubated with secondary antibody Donkey anti-goat Alexa Fluor Plus 647 (A32849, Thermo Fisher) diluted 1:500 and 10 g/mL Hoechst 33342 in blocking buffer for 1 h at 37 C
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