The disseminated iPSCs were cultured in Neurobasal Medium (GIBCO, Thermo Fisher Scientific, MA, USA) supplemented with 0.5% B27 without vitamin A (GIBCO, Thermo Fisher Scientific, MA, USA), 1 Glutamax (GIBCO, Thermo Fisher Scientific, MA, USA), 2 mg/mL doxycycline hydrochloride (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), and 5 mM Y-27632 (Nacalai-Tesque, Kyoto, Japan)
Lion King for days
Ferroptotic cancer cells also release danger-associated lipids and iron intermediates that recruit and activate macrophages, creating positive or negative regulatory loops ( 5 Iron homeostasis and immune regulation in the tumor microenvironment 5.1 Impact of macrophage polarization states on iron metabolism During early tumorigenesis, M1-type tumor-associated macrophages (TAMs) facilitate cellular iron uptake via transferrin receptor 1 (TfR1/CD71), while ferritin (FT) restricts iron efflux, resulting in iron sequestration
J.Domnguez-AndrsJ.CurtisN.van CrevelR.van de VeerdonkF
Okeleji O, Singh S, Bedoy A, Ostermann L, Garland KM, Mak PY, Pacheco C, Ayoub E, Veletic I, Angel M, Luther S, Rohde C, Andreeff M
