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Optional: If the lysate is too viscous, add RNase A (10 g/mL) and DNase I (5 g/mL) and incubate on ice for 10-15 minutes

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139,140 Alkyl-adenine DNA glycosylase (AAG) is a critical enzyme that removes methylated DNA bases in BER

Removal of bile duct stones by ERCP with papillotomy is best if undertaken within 24 h of the patients admission and the value of this approach remains in terms of morbidity and mortality in patients with acute biliary pancreatitis and concomitant cholangitis [209, 210]