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The resin was washed by 20 column volumes of wash buffer A [20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) glycerol, 0.05% (w/v) DDM, 0.01% (w/v) CHS and 30 mM imidazole] and 10 column volumes of wash buffer B [20 mM HEPES, pH 7.5, 500 mM NaCl, 2% (w/v) glycerol, 0.02% (w/v) DDM, 0.01% (w/v) CHS and 50 mM imidazole], followed by incubation with Fab7F38 at a molar ratio of 1: 1.5 in 3 mL wash buffer C [20 mM HEPES pH 7.5, 500 mM NaCl, 2% (w/v) glycerol, 0.01% (w/v) DDM, 0.01% (w/v) CHS and 20 mM imidazole] for 3 h at 4 C

The mechanism is sound

Ipamorelin is a synthetic ghrelin / GHS-R1a receptor agonist (pentapeptide)

CAS Number: 307297-39-8 Molecular Formula: C 14 H 22 N 4 O 9 Molecular Weight: 390.35 g/mol Sequence: Ala-Glu-Asp-Gly (AEDG) Synonyms: Epitalon, Epithalone, AEDG peptide, Khavinson tetrapeptide Mechanisms proposed in research literature: Telomerase (hTERT) induction

Rajesh Dhirmalani serves as a Board-Certified Gastroenterologist, bringing digestive health expertise