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The TUNEL enzyme solution was then added, and after 60 min at 37 C in a dark, humid chamber and DAPI labeling, TUNEL-positive cells were visualized as red fluorescence nuclei at 580 nm (using a Confocal Laser Scanning Microscope

2002 Aug;77(8):754-9.PMID: 12173710 Ashfaq S, Abramson JL, Jones DP, Rhodes SD, Weintraub WS, Hooper WC, Vaccarino V, Harrison DG, Quyyumi AA