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RT-qPCR was performed using the TB Green Premix Ex Taq Kit on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) per the following conditions: Denaturation at 95 C for 30 s
10.1021/acsnano.8b09387 43 LiuJ.WuM.PanY.DuanY.DongZ.ChaoY.et al (2020)
The pain can become so severe that the simple act of cloth touching the area in unbearable
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