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For semaglutide users specifically, the constipation problem is compounded by delayed gastric emptying
Cells were harvested by centrifugation and lysed in buffer containing 50 mM Tris-HCl (pH 8.0), 1 M NaCl, 25 mM imidazole, 0.5 mM -mercaptoethanol, 1 mM PMSF, and protease inhibitors
Semaglutide GLP-1 injections were originally developed to treat individuals with type 2 diabetes
None were already taking diabetes medicines
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