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25,29 In GIP, there is a small chance that activity can be influenced by replacing Ala 13 with Aib
In cell-based assays retatrutide is deliberately unbalanced relative to the native hormones about 0.4 times as active at the GLP-1 receptor, 0.3 times as active at the glucagon receptor and roughly 8.9 times as active at the GIP receptor so GIP signalling carries the most weight while the glucagon arm stays measured [6]
Most importantly, it does not affect the folding stability of the conjugated protein
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