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At 6 h post inoculation, 1 OD bacteria were collected and washed twice by Ringers solution

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Proteins were boiled with Laemmli sample buffer (Biorad) plus 2-mercaptoethanol as loading buffer

NAD-specific glutamate dehydrogenase of Neurospora crassa

18 This observation shifts the discussion beyond descriptive cell biology and suggests that ferroptotic propagation may be encoded, at least in part, in the physicochemical properties of oxidized lipid bilayers rather than in cell-specific signaling programs