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Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) characterizes stepwise bile acid perturbations across DKD stages (199), demonstrating research value but constrained routine applicability given costs exceeding $800 per analysis

But much more research needs to be done

Although the maximum plasma concentration is lower when compound 2 is administered PO (0.77 M) instead of IP (4.8 M), it is still 4.3-fold higher than LD 50 for ex vivo NTS determined in the commonly used 72 h assay, also indicating that TGR inhibitor 2 is orally bioavailable and the initial prediction of oral bioavailability based on the Lipinski et al

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