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19 , e1010387 (2023)

Human embryonic kidney 293 cells containing SV40 large T-antigen (HEK293T) were cultured in DMEM (Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco), 1 mM sodium pyruvate (Gibco) and 100 units/mL penicillin and 100 g/mL streptomycin at 37 C in 5% CO 2

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That single-pathway focus is one reason Ozempic became such a major category-defining drug