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Tuy nhin, nu tiu th qu nhiu protein, c th c th gp kh khn trong vic chuyn ha v loi b cht thi protein qua thn, c bit i vi ngi c vn v thn

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NS5B polymerase was incubated with HCV3END10A RNA in the presence of FAD, ATP, CTP, UTP (complemented with -32P-labeled UTP) and either GTP or 3dGTP

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Ent2 complex was crystallized with a 2:1 (protein:precipitant) ratio in a 0.2 M lithium sulphate, 0.1 M Bis-Tris pH 5.5, 25%(w/v) PEG 3350 solution at 19C