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the second aliquot is chemically reduced before derivatisation which leads to the detection of the sum of both, oxidised and reduced glutathione

demonstrated that in vitro treatment of Eosinophilic leukemia cell line-1 cells (EoL-1) with kaempferol reduced IL-8 levels and Caspase-1 activity

Quinto CA, Mohindra P, Tong S, Bao G

If a reduction in the amount of glucose-6-phosphate dehydrogenase or alteration of structure occurs due to G6PD gene mutations, the enzyme loses its protective role, leading to the accumulation of reactive oxygen species and thus damaging red blood cells