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A key challenge in studying native GLP-1 is its extremely short in vivo half-life of approximately 12 minutes, owing to rapid inactivation by dipeptidyl peptidase-4 (DPP-4), which cleaves the N-terminal His-Ala dipeptide from GLP-1 to produce the inactive metabolite GLP-1(936)amide

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This helps to minimize the counter-regulatory action of GH against higher daytime insulin levels (and its potential for increasing insulin resistance)

Male mice were treated with semaglutide or vehicle, and 4 hours later, the renal artery and kidney were obtained for analysis of protein content

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