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Total RNA of HBE cells and lung tissues were extracted using a GenElute TM Mammalian Total RNA Miniprep Kit (Sigma, U.S.) according to the manufacturers protocol, and the concentration of total RNA was determined by measuring the absorbance at 260 nm using a Nanodrop 2000c spectrophotometer (Thermo Scientific, U.S.)

It is sold in weekly subcutaneous injections at five doses from 2.5 mg through 15 mg, and the Phase 3 SURMOUNT trials showed average weight loss of roughly 20 to 22 percent at the highest dose over 72 weeks

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Dev Med Child Neurol 1991;33:795-802