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Sample size was chosen empirically based on our previous experience in the calculation of experimental variability
The selectivity is documented in the foundational paper: a peer-reviewed European Journal of Endocrinology study demonstrating that Ipamorelin stimulates growth hormone selectively without elevating ACTH, cortisol, or prolactin, which is exactly why it has become the default selective secretagogue in modern peptide medicine
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doi: 10.1038/nm.2123 131
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