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It does this by amplifying Brain-Derived Neurotrophic Factor (BDNF) a natural protein often called Miracle-Gro for the brain. BDNF encourages the growth of new synapses and dendrites, the communication points that connect one neuron to another

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Actin staining was then performed with pre-conjugated Phalloidin-Alexa Fluor 488 (Cytoskeleton, PHDG1A) diluted 1:140 in Antibody Diluent Reagent Solution (Invitrogen, 003218) for 30 min in the dark at room temperature