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Then the cells were washed with PBS, and apoptotic cells were stained with Annexin V-FITC (Ex/Em: 488/519 nm) and propidium iodide (Ex/Em: 535/617 nm) according to the instructions of the kit manufacturer and detected by flow cytometry (BD LSRFortessa, BD Biosciences, USA)

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International Brazilian Journal of Urology: Official Journal of the Brazilian Society of Urology, 44 (4), 794-799

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