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Wound healing assay Isolated PSCs were cultured in 6-well plates for 72 h

However, the physiological linkage between different transcription factors and ROS overexpression showed regulation by the estrogen receptor of antioxidative stress enzymes (16), the molecular target genes of these transcription factors at different stages of the seminiferous epithelial cycle are largely unknown and this shall provide an unprecedented opportunity for further investigation in the field

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Blood samples were collected and glutathione levels determined

The same cell densities were used analogous to 3D liver models