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OPCs in CALs exhibited only downregulation of pathways, including cholesterol homeostasis, interferon signaling, and fatty acid metabolism, whereas OPCs in RLs showed upregulation of hypoxia, glycolysis, and TNF signaling pathways (Supplementary Fig

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Tools such as DeepCRISPR and CRISPR-Net exemplify this transition toward predictive editing design by enabling the identification and prioritization of target sites with reduced off-target risk before experimental implementation (Chuai et al., 2018