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To confirm this hypothesis, UFL1 was depleted in MCF-7 (Luminal A), BT474 (Luminal B), SK-BR-3 (HER2 + ), MDA-MB-231 (TNBC), and MDA-MB-468 (TNBC) breast cancer cells using two independent shRNAs targeting UFL1 (shUFL1#1 and shUFL1#2) for cell growth assays
Three years into taking TRT, he overhauled his diet (he aims for 225 grams of protein daily), started strength training six days per week, cut his alcohol intake by 90 percent, and began a low dose of a GLP-1
10.1592/phco.30.5.463 133 NicolausM.BrdlJ.LinkeR.WoerleH