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Vitamins and anti-inflammatory herbs: May have helped, but I took them over the course of two years and continued to experience pain and stiffness

Daytime energy improves

ER and Golgi stain were performed in Triton 0.1%, 20 min, RT

IV infusion, though fully absorbable, is only advised under medical supervision due to potential risks

Parasite pellets were resuspended in buffer (3.5 mM MgCl 2 , 110 mM KCl, 40 mM NaCl, 20 mM HEPES, 6 mM EDTA, pH 7.4) with protease inhibitors (0.01 mg of leupeptin A, 0.001 mg of pepstatin A, 0.35 mg of PMSF) lysed by three freeze/thaw cycles (liquid nitrogen and 37C water bath), and protein content was determined using Bio-Rad DC Protein Assay