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The medication's potency could be affected: Leaving drugs that require refrigeration at room temperature or higher might hasten the deterioration of their active components and decrease their effectiveness even if it is within their beyond-use-date (BUD) 3
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The supernatant containing the proteasomes were separated after centrifugation at 12,000 xg for 15 min at 4 C and were quantified and diluted to 1 g/L concentration with proteasome lysis buffer
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