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Preparation Condition 3 (EDTA 80 o C deproteinization): Deproteinization may not be necessary to prevent spontaneous oxidation of free GSH Unexpectedly, we found no difference in the levels of total GSH, free GSH, GSSG, or the ratio of free GSH to GSSG between the EDTA-treated plasma samples which were stored at 80 o C (Preparation Condition 2 and Preparation Condition 3)
It is not yet fully understood the mechanisms underlying BBB protection, but studies indicate it may be through a CD14-depenent mechanism, suppressing expression of LRP1, and protection from oxidative stress [146]
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Bondanza, S
It activates longevity pathways such as AMPK and reduces oxidative stress
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