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For highest resolution, Airyscan detection was used with the following settings: 488 nm, 561 nm and 640 nm excitation with emission bands of 492549 nm, 564611 nm and 644700 nm and images processed using the Airyscan joint deconvolution algorithm

After the injection, the needle should be safely disposed of in a sharps container, which we can provide for you

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Optimize your injection technique Where and how you inject affects absorption