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researchers often allow the solvent a short equilibration period and swirl gently rather than vortexing vigorously to bring the full quantity into solution

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The effluent was ionized via ion electrospray in negative mode on a TSQ Quantiva triple-quadrupole mass spectrometry with the following instrument settings: ion spray voltage 2500 V, sheath gas 28, ion transfer tube temperature 325, aux gas 8, and FWHM of 0.7 for both Q1/Q3 resolution

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