After passing through the 70 m cell strainer (BD Falcon, Bedford, MA, United States), the cell suspension was centrifuged at 300 g for 5 min
They are found in things we use everyday: plastic water bottles, makeup, hair care products, receipts, carpet, non-organic foods, over-the-counter pain relievers, and a multitude of other things
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Typically, the E3 ubiquitin ligase complex comprised of Kelch-like ECH-associated protein 1-Cullin 3-Ring box 1 (KEAP1-CUL3-RBX1), S-phase kinase associated protein 1-Cullin 1-Rbx1/-transducin repeat-containing protein (SCF/-TrCP), and synonymin/Hrd1, continuously degrades Nrf2 proteasomes, maintaining Nrf2 at a low expression level [64]
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