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The discrimination among the substrates almost exclusively favor 5-AD for HsaGST A3-3 and EcaGST A3-3, whereas trans -2-nonenal is a signature substrate for HsaGST A4-4

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The best binding poses for both substrates had a highly similar conformation to ()-catechin ( 12 ), with an RMSD of matched atoms of 0.51 for flavan-3,3,4-triol ( 4 ) and 0.54 for flavan-3-on-4-ol ( 7 )

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Acknowledgements We thank the EMBL Gene Core facility for help with the 16S amplicon sequencing