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Dissections were performed according to the atlas of RNA preparation and gene expression analysis by quantitative real-time PCR Total RNA was isolated by a single step of guanidinium isothiocyanate/phenol extraction using PureZol RNA isolation reagent (Bio-Rad Laboratories, Segrate, Italy) ( Cytochrome c oxidase 1 ( Cox1 ) (Rn03296721_s1), Cox3 (Rn03296820_s1), Catalase ( Cat ) (Rn00560930_m1), and Glutathione peroxidase 1 ( Gpx1 ) (Rn00577994_g1, Thermo Fisher Scientific, Monza, Italy) mRNA levels

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