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iPS cell colonies were analysed by alkaline phosphatase staining according to the manufacturers protocol (AP blue membrane substrate detection kit
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PCR data showed that 200 M H 2 O 2 treatment upregulated IL-1, MMP13, Collagen-X, Caspase3, and Caspase9 at mRNA level in chondrocytes, while metformin significantly attenuated these H 2 O 2 exposure induced increases (Fig
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These simulations utilized the lowest-energy solution of the NMR-derived ensemble of the p53 TAD2 -FOXO4-DRI complexes as our starting geometry (see methods)
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