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AMPK activation increases PKA activity, while PKA independently or in turn inhibits the activity of Ser / Thr -specific protein phosphatase-2C, thereby indirectly modulating AMPK activity in rat primary vascular smooth muscle cells 41

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Blood was flushed out with 150 mL of saline with 100 U/L heparin at 30 mL/min, followed by perfusion with 3% formaldehyde and 150 mL of 1% glutaraldehyde solution