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Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions

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[4] Graf MV, Kastin AJ, Coy DH, Fischman AJ

This vascular running phenomenon essentially upgrades minor vessels to assume major circulation roles when needed