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These were allowed to adhere overnight in full culture media (DMEM (41965039) with 4 mM L-glutamine, 10% dFBS (26400044), 1 mM sodium pyruvate (11360039) and 50 U ml 1 penicillinstreptomycin (15070063)) under normal cell culture conditions

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Diagnosis typically involves assessment of risk factors, blood tests (which may show elevated liver enzymes such as ALT and AST, though many people with NAFLD have normal liver blood tests), and imaging studies such as ultrasound or transient elastography (FibroScan)

Fluorescent colonies of C