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Standard centrifugation failed to pellet the small (~80 nm) liposomes effectively without ultra-high speeds that damaged the vesicles
This isn't a guidelineit's the temperature at which the lyophilised powder remains stable for the durations specified by manufacturers, typically 2436 months from synthesis
These changes, or reactions, can vary in type and severity
Mike points out from multiple studies that multi-set protocols are far more beneficial than single-set protocols
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