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Aortic tissue LOXL2 activity assay Aortas from young ( 18 months old) WT and LOXL2+/ littermates were extracted and cleaned free of connective tissue in Krebs-HEPES buffer (NaCl 135 mM, KCl 5 mM, MgSO 4 1 mM, K 2 HPO 4 0.4 mM, Glucose 5.5 mM, HEPES 20 mM, adjust the pH to 7.4, supplement with 1 mM CaCl 2 ) containing 0.25 g/mL fungizone (Gibco)

Proteins without his tag were purified by affinity chromatography either on glutathione 21 (GSTs A4-4, M1-1, M2-2 and M5-5) or on S-hexyl-glutathione 22 (GSTM4-4) linked to epoxy-activated Sepharose 6B (GE Healthcare)

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The bioavailability of regular, non-liposomal glutathione is very low since it is susceptible to oxidation and used as a cofactor in many metabolic reactions

Avoid using solutions of vitamin B containing benzyl alcohol in neonates, as gasping syndrome may occur