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Twenty-six hours post-infection, stable pools of cells were selected with 1.2 g/mL puromycin (Invitrogen, San Diego, USA) for MCF-7 cells and 1.4 g/mL puromycin for ZR-75-1 cells for 3 days or 100 g/mL hygromycin for MCF-7 and ZR-75-1 cells for 7 days before being used for subsequent experiments

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Sulforaphane ameliorates the development of experimental autoimmune encephalomyelitis by antagonizing oxidative stress and Th17-related inflammation in mice