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Complex was formed by adding 10 mM MgCl 2 , 1 mM MnCl 2 , 50 mU/mL apyrase, 30 M tirzepatide, 100 M TCEP and 10 g/mL Nb35 to the cell lysate and incubated at RT for 1.5 h
The REDEFINE-4 head-to-head result (23% vs 25.5% for tirzepatide) matters
The top binding poses were evaluated in PyMOL based on hydrogen bonding, hydrophobic interactions, and binding energy
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