Interestingly, resistance training appears to increase IGF-1 activity, at least in middle aged women
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MTT assay After a total of 7.5 10 3 cells were seeded onto 24-well plate, the cytotoxic effects of EE on C2C12 myoblasts were assessed 24, 48, and 72 hs after incubation with various concentrations (1, 10, 100, 500, and 1000 g/ml) of EE
Experimental methods Chemicals and reagents High-performance liquid chromatography-grade methanol, water, and acetonitrile were purchased from J.T
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