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Although PTX exerts significant cytotoxic effects in the treatment of advanced GC, a considerable proportion of patients develop acquired resistance to PTX during administration, ultimately leading to treatment failure 46,47

Western blot analysis Protein lysates were obtained by homogenizing tissues or cell pellets in sample buffer containing 62.5 mM Tris-HCl (pH 6.8), 4% SDS, 200 mM dithiothreitol (DTT), 10% glycerol, and 0.001% bromophenol blue using a tissue to buffer ratio of 1:10 (w/v), followed by boiling

A 10-week MeDi intervention in World Trade Center responders with PTSD significantly reduced inflammatory markers such as CRP and lessened PTSD symptom severity compared to standard nutritional advice ( Eubacterium eligens , a short-chain fatty acid producer from dietary fiber, coinciding with lower PTSD severity, indicating that Mediterranean dietary patterns may support gut-mediated neuroprotection (Ke et al., 2023)

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Following the development of motor symptoms, cognitive dysfunctions progress due to the involvement of the atemporal cortex (Kalia 2018)