Adding more water to the same vial makes each dose a larger, easier-to-measure liquid volume
You get the ritual, you still get the taste, just without the hangover
Under normal conditions, Nrf2 binds to Keap1 in the cytoplasm, resulting in proteasomal degradation
ATP-based luminescence assays, such as the CellTiter-Glo 2.0 Assay, are the preferred platform for HTS drug screening because they offer the highest sensitivity (detecting 15100 cells/well), the simplest protocol (single reagent addition, 10-minute read time), and the lowest coefficient of variation across plate formats
Krutkin DD, Thomas S, Zuffa S, Rajkumar P, Knight R, Dorrestein PC, et al