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(ii) GSH exerts a concentration-dependent effect on NF-YA1 gene expression, which was downregulated in (h)GSH-depleted wild-type and sym33-3 roots with nodules and upregulated in both genotypes following treatment with the low (0.1 mM), but not high (1 mM) concentration of GSH
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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation
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