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Additionally, a randomized, double-blind, placebo-controlled trial in 2,588 healthy older adults with mild hyperhomocysteinemia found that daily supplementation with 500 g of vitamin B 12 and 400 g of folic acid for two years reduced blood homocysteine concentrations but had no benefit on depressive symptoms in those with or without depressive symptoms at baseline (110)

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using a higher volume produces a more dilute solution, which may be easier to measure accurately

[1] A frequently employed regimen involves applying approximately 1-2 mL of the 0.5 % solution to affected scalp regions once to twice daily, preferably on clean, dry skin in the evening