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Longer travel requires portable cooling solutions such as insulated bags with ice packs or mini refrigerators

Longer-standing issues and partial tears may need six to eight weeks of consistent treatment

All data were analyzed using an Eppendorf Realplex 2 and the relative expression levels were calculated using the 2 CT method

For longer storage, some researchers freeze aliquots at -20 degrees Celsius, though repeated freeze-thaw cycles degrade peptides